The invention discloses an analytic method of cuticular hydrocarbons of the larvae of fruit flies, belonging to the field of inspection and quarantine. The analytic method comprises the following steps: cutting open the whole body of a larva of fruit flies along the dorsopleural line from the head or anus at the end of the abdomen, eliminating the content of the body, rinsing clean, adding a proper amount of an organic solvent, quickly and fully grinding and extracting for 1-4min, then adding an equal amount of organic solvent, leaching for 1-5min, collecting all the leaching solution, and performing GC-MS analysis after column separation. The method disclosed by the invention improves the related analytic methods of cuticular hydrocarbons of the larvae of fruit flies in the prior art, and shortens the whole detection time from 12-24h originally to 2-3h, thus greatly improving the timeliness. In addition, the method disclosed by in addition can acquire more kinds of cuticular hydrocarbons by leaching, thus providing detailed and reliable data supports for further judging the species especially allied species of fruit flies.