The invention provides an optical pump magnetometer gradient tolerance measuring device. The optical pump magnetometer gradient tolerance measuring device mainly comprises a horizontal base, a gradient generating coil, a horizontal connecting rod, bubble levels, a connecting rod leveling device, an optical pump probe mounting clamp, a laser range finder, a precise current source and a measuring and control module, wherein the measuring and control module calculates the magnitude of currents which need to be output to generate a specific magnetic gradient according to preset parameters such as the probe position, probe size, coil turn number and coil radius and controls the precise current source through a USB interface to output specific currents to the gradient generating coil; the gradient generating coil is mounted on the horizontal base, the position of the gradient generating coil is adjusted through the bubble level A, and the gradient generating coil generates the magnetic gradient in a surrounding space; the optical pump probe mounting clamp is arranged on the horizontal connecting rod, and the horizontal connecting rod is regulated through the bubble level A, the bubble level and the connecting rod leveling device to be parallel with the axis of the gradient generating coil.