The invention relates to a factorized high-efficiency mushroom production culture material and a preparation method thereof. The factorized high-efficiency mushroom production culture material is prepared from the following ingredients by weight percent: 70 to 80% of saw dust, 10 to 20% of bran and 1 to 10% of gypsum, wherein the saw dust includes first-level saw dust, second-level saw dust and third-level saw dust which has different granularities. The preparation method comprises the steps of fully mixing the raw materials according to a proportion and then adding water to evenly stir. The factorized high-efficiency mushroom production culture material disclosed by the invention optimizes the matching of saw dust in a factorized mushroom production formula, improves spawn running speed of hypha in factorized mushroom production, shortens a factorized production period, improves mushroom yield and quality, improves biotransformation efficiency of factorized production, increases earnings of a producer and has good application prospect.