The invention discloses a method for rapid screening and cultivation of anti rust fungus Hemerocallis germplasm. Hemerocallis seeds harvested after same-year hybridization of parent Hemerocallis are immediately refrigerated for 30-60 days at 4-8 DEG C; then the seeds are sown, planting environment is a greenhouse with artificially controlled temperature and humidity, and the temperature is 15-30DEG C; after the emerocallis hybrid seeds are germinated, and grown to seedlings of 10cm, a pathogenic spore suspension is began to be sprayed for the first time, the pathogenic spore spraying concentration is controlled in 10<6> spores to10<7> spores / ml, after the spraying, the seedlings are covered with a plastic film for preserving moisture for 3 days, then the plastic film is removed, the pathogenic spore suspension is sprayed once every 30 days, the pathogenic spore suspension is totally sprayed for 3 times, before each time of spraying, 0.5-1cm of the top ends of all the seedlings are cut by a pruning shear in order to manufacture wounds to help pathogen spore invasion. The whole process of the method is manually intervened, and the method has the advantages of directional breeding, definite target, strong operability, simple implementation and stable and reliable results.