The invention provides a preparation method of a Yangchunyuye soup. The method comprises the following steps of (1) mixing and extracting pilose antler and tortoise shell, and performing concentration, alcohol precipitation and filtering on extract liquor to obtain precipitates and filter liquor 1; (2) adding antler glue into the precipitates, and performing heat-melting, acid hydrolysis and filtering to obtain a component 1; (3) extracting herba epimedii through water, and concentrating and filtering extract liquor to obtain a component 2; (4) mixing and extracting codonopsis pilosula, lyceum barbarum, morinda officinalis, asparagus fern, fructus cnidii and prepared rehmannia root, and performing concentration, alcohol precipitation and filtering to obtain filter liquor 2; (5) combining the filter liquor 1 and the filter liquor 2, and performing alcohol precipitation and filtering to obtain a component 3; (6) combining and filtering the components of 1-3. By means of hot-reflux extraction and separate extraction of the herba epimedii, the preparation method of the Yangchunyuye soup can improve the content of effective components in products, ensure environmental protection and energy conservation during production and further reduce the cost.