The invention discloses a method for extracting phytol from coccolithophores. The method comprises the steps of using ethyl acetate to conduct extraction; using an antiphase silica gel column to conduct primary chromatography; using the antiphase silica gel column to conduct secondary chromatography; using a sephadex column LH-20 to conduct chromatography; using a normal phase silica gel column to conduct chromatography; extracting phytol from coccolithophores through a PTLC preparing method. According to the method for extracting phytol from coccolithophores, phytol of coccolithophores ethyl acetate phase extracts is separated and purified for the first time, and research is made on cytotoxicity of phytol, so that a basis is laid for subsequent large-scale production of phytol.