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发明公开
CN107142312A 一种快速检测工业巴氏酵母芳香醇代谢基因的方法 有权

一种快速检测工业巴氏酵母芳香醇代谢基因的方法
摘要：
本发明涉及生物工程技术领域，尤其涉及一种快速检测工业巴氏酵母芳香醇代谢基因的方法，包括总RNA的提取、逆转录反应制备cDNA模板、多重PCR反应、毛细血管电泳、产物片段分析，针对工业巴氏酵母芳香醇代谢相关基因ADH1‑Sc，ADH1‑Sb，ADH3‑Sc，ADH3‑Sb，ADH4‑Sc，ADH4‑Sb，ADH5‑Sc，ADH5‑Sb，ARO8‑Sc，ARO9‑Sc，ARO9‑Sb，ARO10‑Sc，ARO10‑Sb，ARO80‑Sc和ARO80‑Sb。本发明所采用的GeXP多重基因表达定量分析技术具有更强的特异性和灵敏性、自动化程度高等特点，保证了结果的可靠性和可重复性，大大缩短了实验时间。本发明有利于在不同生产条件下，控制发酵过程中芳香醇代谢及含量，提高啤酒的典型风味及一致性；并可以通过快速调整啤酒中芳香醇类风味，突出产品特色及进行新产品的开发。
摘要（英）：

The invention relates to the technical field of bioengineering technology, in particular to a method for rapidly detecting metabolizing genes of industrial aromatic alcohol of saccharomyces pastorianus. The method includes the steps of extraction of total RNA, reverse transcriptional reaction for preparation of cDNA templates, multiple PCR reactions, blood capillary electrophoreses, analyses of product sections, and metabolism of relative genes specific to industrial aromatic alcohol of saccharomyces pastorianus, wherein the relative genes include the follows: ADH1-Sc, ADH1-Sb, ADH3-Sc, ADH3-Sb, ADH4-Sc, ADH4-Sb, ADH5-Sc, ADH5-Sb, ARO8-Sc, ARO9-Sc, ARO9-Sb, ARO10-Sc, ARO10-Sb, ARO80-Sc and ARO80-Sb. The GeXP multiple-genetic-expression quantitative-analysis technology adopted in the method has the characters of higher specificity, sensitivity, an excellent automation degree and the like, and ensures the reliability and repeatability of results and extremely decreases experimental time. The method for rapidly detecting metabolizing genes of industrial aromatic alcohol of saccharomyces pastorianus has the advantages of facilitating control of metabolism and content of aromatic alcohol during a fermentation process under different production conditions, improving typical flavor and consistency of beer, and being capable of highlighting product features and conducting development of new products by rapidly adjusting flavor of aromatic alcohol inside beer.

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