The invention discloses kiwi fruit seed polyphenol antibacterial soap and a preparation method of same. The antibacterial soap is composed of, by mass, 9-10 parts of kiwi fruit seed oil, 6-7 parts of palm oil, 6-7 parts of olive oil, 5-6 parts of wool fat, 18-22 parts of sodium hydroxide, 20-25 parts of distilled water, 4-6 parts of sodium dodecyl sulfate, 1-2 parts of trehalose, 2-3 parts of sodium chloride, 2-3 parts of sucrose, 9-10 parts of glycerol, 2-3 parts of propylene glycol, and 1-4 parts of kiwi fruit seed polyphenol. The antibacterial soap is prepared with the kiwi fruit seeds as a raw material, so that comprehensive utilization rate of byproducts in processing of kiwi fruits is increased. The antibacterial soap has inhibiting effect on Bacillus subtilis, Staphylococcus aureus, Bacillus cereus and Escherichia coli. The invention solves a problem that antibacterial soap in the prior art contains chemical synthesis substances and is harmful to body health and environment.