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发明公开
CN107201349A 一种表达菜豆环氧化物水解酶的工程菌及应用 无效 - 驳回

一种表达菜豆环氧化物水解酶的工程菌及应用
摘要：
本发明公开了一种表达菜豆环氧化物水解酶的工程菌及应用，属于生物工程技术领域。本发明提供了一种环氧化物水解酶及表达该酶的重组大肠杆菌。本发明的环氧化物水解酶能够催化外消旋对氯环氧苯乙烷(rac‑pCSO)生成(R)‑对氯苯基乙二醇(pCPED)，在转化率达99.7％时产物对映体过剩值为85.1％eep。与其它植物来源的环氧化物水解酶相比与提高了10倍。
摘要（英）：

The invention discloses an engineering strain for expressing epoxide hydrolase of kidney beans, and application of the engineering strain, belonging to the field of biological engineering technology. The invention provides the epoxide hydrolase and recombinant escherichia coli for expressing the epoxide hydrolase. The epoxide hydrolase provided by the invention can catalyze racemic p-chloro-epoxyethylbenzene (rac-pCSO) to generate (R)-p-chloro-phenyl ethylene glycol (pCPED); when the conversion rate reaches 99.7%, an enantiomer excess value of the product is 85.1%eep. Compared with other plant-derived epoxide hydrolases, the epoxide hydrolase provided by the invention increases the value by 10 times.

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