The invention relates to a production method of isolation fabric, particularly to a production method of fireproof and waterproof isolation fabric. The production method comprises the steps as follows: step 1, twill nylon silk is washed with industrial acetone and aired after being washed for later use, and vulcanized silicone rubber, tetraethoxysilane and dibutyltin dilaurate are polymerized into a glue liquid; step 2, the washed twill nylon silk is placed in the glue liquid, taken out after being fully soaked in the glue liquid and suspended and aired to be naturally vulcanized. The isolation fabric has good fireproof and waterproof functions and can be used as outer fabric of airplanes as well as special textile with fireproof and waterproof requirements in air plane cabins. The isolation fabric takes 106 silica gel with low surface activity as a coating and thus has good stick resistance, can be used as non-stick backing of various glue films and can be easily stripped during usage.