The invention discloses a peak-load regulation resource call decision-making method suitable for large-scale renewable energy grid connection, and the method comprises the following steps: obtaining a time sequence load demand PL in a specific time period in a specific region, and determining a peak-load regulation demand At of a power dispatching mechanism; determining the time sequence prediction output PW of a wind turbine generator set and the time sequence prediction output PS of a solar generator set; determining usable peak-load regulation resources Mi in a system, determining the adjustable measurement range of each type of peak-load regulation resources Mi, the peak-load regulation capacities DMi, and the corresponding peak-load regulation costs CMi, and constructing a mixed integral optimization model; and obtaining a peak-load regulation resource call sequence optimized in the specific time period. Through the construction of an economic model and a cost model of each peak-load regulation means, the method achieves the construction of the mixed integral optimization model which takes the economical efficiency as the target. Through model solving, the method obtains the peak-load regulation resource call quantity and sequence with the optimal cost effectiveness, enables a power system to adapt to the efficient consumption of renewable resources, improves the peak-load regulation capability of the power grid, and is better in practical guide significance and application value.