The invention provides a selective culture medium for skin fibroblast culture. The selective culture medium comprises a basic culture medium, fetal calf serum, human serum albumin, bovine pituitary extracts, insulin, vitamin C, biotin, insulin-like growth factors and epidermal growth factors which are mixed according to a proportion being 1ml to (0.0125ml to 0.05ml) to (0.005ml to 0.025ml) to (80mug to 423mug) to (7mug to 18mug) to (22mug to 60mug) to (37mug to 214mug) to (1mug to 20mug) to (0.1mug to 1mug). A DMEM culture medium and an F12 culture medium are used for basic culture. The selective culture medium for skin fibroblast culture has the advantages that the selectivity is realized on the fibroblast; the purity of the fibroblast is improved; the sundry cell pollution is avoided; the cell passage number is effectively reduced; the cell proliferation speed is high. The selective culture medium provided by the invention can also reduce the serum consumption; the cost is reduced.