The invention discloses a system and a method for animal robot autonomous navigation. The system comprises a GPS module arranged on a controlled animal, a multi-channel neural signal stimulation module and a control module. The GPS module is configured to receive a GPS satellite signal, and outputs current position information of an animal robot to the control module. The control module is configured to receive the current position information of the GPS module, and compare the current position information with set target position information, to determine a navigation path and form a control instruction. The multi-channel neural signal stimulation module is configured to include a mirror current source, a boost circuit, and an analog multiway switch. Through the connected mirror current source and the boost circuit, constant current is obtained. According to the control instruction, a corresponding channel of the analog multiway switch is selected to output constant current which acts on a neural control region of the controlled animal, to realize output of simulation neural coding information and realize navigation.