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CN107446924B 一种基于CRISPR-Cas9的猕猴桃基因AcPDS编辑载体及其构建方法和应用 失效 - 权利终止

一种基于CRISPR-Cas9的猕猴桃基因AcPDS编辑载体及其构建方法和应用
摘要：
本发明公开了一种基于CRISPR‑Cas9的猕猴桃基因AcPDS编辑载体及其构建方法和应用。本发明建立的基于CRISPR‑Cas9的猕猴桃基因AcPDS编辑载体能够快速简单地对猕猴桃基因进行高效的定点突变，弥补了猕猴桃中基因定点编辑技术的空白。实验结果证明：通过农杆菌介导的猕猴桃遗传转化，本发明的基于CRISPR‑Cas9的猕猴桃基因AcPDS编辑载体成功地对猕猴桃基因AcPDS的两个靶点进行了定点突变，同时引起了白化的表型。
摘要（英）：

The invention discloses a compiling vector of a kiwi fruit gene AcPDS based on CRISPR-Cas9 as well as a construction method and application thereof. The compiling vector of the kiwi fruit gene AcPDS based on CRISPR-Cas9 established can quickly and simply perform efficient site-specific mutagenesis on the kiwi fruit gene, so that the blank of a gene fixed point compiling technology in kiwi fruit is filled. Experimental results verify that by means of agrobacterium-mediated kiwi fruit genetic transformation, the compiling vector of the kiwi fruit gene AcPDS based on CRISPR-Cas9 performs site-specific mutagenesis on two target spots of the kiwi fruit gene AcPDS successfully, and meanwhile, an albefactedphenotype is induced.

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