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发明公开
CN107475212A 分泌表达葡萄糖氧化酶的方法、重组菌及其应用 审中-实审

分泌表达葡萄糖氧化酶的方法、重组菌及其应用
摘要：
本发明涉及分泌表达葡萄糖氧化酶(GOD)的方法、重组菌及其应用，揭示一种能够有效促进GOD表达、提高其酶活的方法，通过在酵母菌中共表达GOD基因和PDI1基因或PDI3基因来实现。同时，本发明还提供了以毕赤酵母(Pichia pastoris)为宿主构建的高效分泌GOD的重组菌。
摘要（英）：

The invention relates to a method for secretorily expressing glucose oxidase, and recombinant bacteria and applications thereof, and discloses a method capable of effectively promoting GOD expression and improving the enzyme activity. The method can be realized by co-expressing a GOD gene and a PDI1 gene or a PDI3 gene in saccharomycetes. Furthermore, the invention further provides efficiently secreted recombinant bacteria constructed by taking Pichia pastoris as a host.

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