The invention discloses a current type PUF circuit for utilizing a reference current source. The current type PUF circuit comprises an input register, a reference current source, a bias current comparator and a time sequence controller, the input register is used for guaranteeing that excitation signals are synchronous and avoiding that asynchronous excitation signals influence an output response,the reference current source generates a reference current with temperature and voltage compensation, a bias current source array generates two bias currents controlled by the excitation signals, thebias current comparator generates judgment output according to the magnitude of the bias currents provided by the bias current source array, and the time sequence controller is used for generating time sequence information of work of the PUF circuit. The current type PUF circuit for utilizing the reference current source has the advantages that the bias current source array outputs the bias current with high robustness; the PUF circuit has high reliability; experiment results show that the PUF circuit has good uniqueness, and the reliability of work at different temperatures (-40 to 120 DEG C) and voltages (1.08-1.32 V) reaches up to 99.6%.