The present invention relates to a microfluidic chip apparatus used for diagnosing syphilis and using polydimethylsiloxane as a substrate, and belongs to the field of analysis and testing. According to the present invention, the use of inexpensive and easily-processed polydimethylsiloxane (PDMS) to produce the substrate of a microfluidic chip for diagnosing syphilis has the following series of problems; and the multichannel chip apparatus is characterized in that the substrate selects the PDMS having an original ecological surface, a hinge type fixture provided with a miniature ultrasonic wavetransducer is manually fastened and positioned at a position close to the sample liquid flow terminal end of the microfluidic chip, ultrasonic waves induce to form the driving force based on interfacial tension difference, the ultrasonic wave induced driving force and the driving force of a micro pump are subjected to coupled operation so as to synergistically drive the sample liquid flow to flowto the terminal direction, the pipeline of the chip is filled with a micro-scale silica particle filler, and the micro-scale silica particle filler is used for supporting the inner wall of the pipeline, compensating the original hydrophobic pipeline with the set of hydrophilic micro-flow channels, and extruding and covering the inner wall surface of the pipeline so as to reduce the opportunity ofthe contact between biological macromolecules and the inner wall surface.