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发明公开
CN107602664A 一种源于鮸鱼鱼鳔的降血脂五肽及其应用 无效 - 驳回

一种源于鮸鱼鱼鳔的降血脂五肽及其应用
摘要：
本发明公开了一种源于鮸鱼鱼鳔的降血脂五肽，本降血脂五肽的氨基酸序列为：Gly-Ile-Glu-Trp-Ala(GIEWA)，ESI-MS测定分子量为574.64Da；将鮸鱼鱼鳔浆液经碱浸提与醇脱脂处理后制得脱脂鮸鱼鱼鳔，在经碱性蛋白酶酶解得鱼鳔酶解液，将酶解液经超滤、离子交换树脂层析、凝胶柱层析、反相高效液相色谱纯化，得到鮸鱼鱼鳔降血脂五肽；本发明同时还公开了一种源于鮸鱼鱼鳔降血脂五肽在降血脂活性成分、保健产品和食品添加剂中的应用。鮸鱼鱼鳔降血脂五肽既能下调成脂基因，又能促进脂肪酸β氧化，减少脂质的生成，具有良好的降脂活性，可开发为降血脂药品、保健产，以及食品添加剂。
摘要（英）：

The invention discloses lipid-decreasing pentapeptide derived from Miichthysmiiuy swimming bladders. The amino acid sequence of the lipid-decreasing pentapeptide is Gly-Ile-Glu-Trp-Ala(GIEWA), and themolecular weight determined by virtue of ESI-MS is 574.64Da. The lipid-decreasing pentapeptide is prepared by the steps of processing the Miichthysmiiuy swimming bladders by virtue of alkali leachingand alcohol degreasing so as to obtain degreased Miichthysmiiuy swimming bladders, carrying out enzymolysis by virtue of alkaline protease, and carrying out ultrafiltration, ion exchange resin chromatography, gel column chromatography and reversed-phase high performance liquid chromatography purification on enzymatic hydrolysate, so as to obtain the lipid-decreasing pentapeptide derived from theMiichthysmiiuy swimming bladders. The invention simultaneously discloses application of the lipid-decreasing pentapeptide derived from the Miichthysmiiuy swimming bladders in lipid-decreasing active components, health products and food additives. According to the lipid-decreasing pentapeptide derived from the Miichthysmiiuy swimming bladders, adipogenesis genes can be reduced, the oxidation of fatty acid beta can be promoted, the generation of lipids can be promoted, and the pentapeptide has good lipid-decreasing activity, and can be developed into lipid-decreasing drugs, health products and food additives.

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