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发明授权
CN107603980B 一种基于PTG-Cas9的猕猴桃基因AcPDS编辑载体及其构建方法和应用 失效 - 权利终止

一种基于PTG-Cas9的猕猴桃基因AcPDS编辑载体及其构建方法和应用
摘要：
本发明公开了一种基于PTG‑Cas9的猕猴桃基因AcPDS编辑载体及其构建方法和应用。本发明建立的基于PTG‑Cas9的猕猴桃基因AcPDS编辑载体能够快速简单地对猕猴桃基因进行高效的多靶点定点突变，弥补了猕猴桃中多基因或者多靶点定点编辑技术的空白。实验结果证明：通过农杆菌介导的猕猴桃遗传转化，本发明的基于PTG‑Cas9的猕猴桃基因AcPDS编辑载体成功地对猕猴桃基因AcPDS的四个靶点进行了定点突变，并引起了白化的表型。
摘要（英）：

The invention discloses a kiwi fruit gene AcPDS editing carrier based on PTG-Cas9 and a construction method and application thereof. The constructed kiwi fruit gene AcPDS editing carrier based on PTG-Cas9 can quickly and simply carry out efficient multi-target-point site-specific mutagenesis on a kiwi fruit gene, and the blank of the kiwi fruit multi-gene or multi-target-point site-specific editing technology is filled up. Experiment results prove that by means of agrobacterium tumefaciens mediated kiwi fruit genetic transformation, the kiwi fruit gene AcPDS editing carrier based on PTG-Cas9 successfully carries out site-specific mutagenesis on four target points of the kiwi fruit gene AcPDS, and causes the albino phenotype.

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