The invention provides a full-automatic detoxified potato minituber aerial-mist cultivation and production device. A clear water injection system, a culture solution injection system and a nutrient solution supply system are arranged between a lower partition plate and the bottom of a box body, an atomization pump of the nutrient solution supply system is connected with a main liquid supply pipe and a branch liquid supply pipe, a magnetic valve is arranged on the branch liquid supply pipe, atomization spray heads are uniformly arranged on the branch liquid supply pipe, culture trays are arranged at the upper portion of the lower partition plate, a plurality of culture holes are formed in the culture trays in an array mode, and a plurality of air adjusting boxes, LED lamps and a humidifierpipeline outlet are arranged between the culture trays and the top of the box body. The full-automatic detoxified potato minituber aerial-mist cultivation and production device can meet the industrialized production demand of a detoxified potato minituber mist culture method, is conductive to growth of potato tubers, is easy to operate and stable in operation and capable of achieving annular production, the operating cost is reduced, a production period is shortened, the breeding speed of breeder's seeds is improved, the production cost is reduced, industrialized production of domestic detoxified potato minitubers is achieved, and conditions are provided for automatic management.