The invention relates to a temperature and humidity uniformity reinforced fuel cell single cell and an electric pile. The single cell comprises a positive electrode plate, a negative electrode plate and a membrane electrode assembly arranged between the positive electrode plate and the negative electrode plate; a plurality of positive electrode flowing channels are formed in the positive electrodeplate in parallel; the gas flowing directions in the adjacent two positive electrode flowing channels are opposite; negative electrode flowing channels are formed in the negative electrode plate; andthe electric pile comprises a plurality of single cells stacked in sequence. Compared with the prior art, the gas flowing directions in the adjacent two positive electrode flowing channels are opposite, so that the conditions of over-low or over-high partial temperature and humidity caused by the single gas flowing direction of the conventional flow field can be effectively relieved, the temperature and humidity difference in a gas inlet and a gas outlet can be reduced, the temperature and humidity distribution uniformity of the electric pile can be improved, and water and heat balance in theelectric pile can be ensured, thereby further improving working performance and use stability of the fuel cell; and in addition, the fuel cell single cell is easy to process and manufacture.