The invention relates to a grifola frondosa facility planting new variety and a preparation method of its liquid fermenting culture, and belongs to a food-medicine mushroom and preparation method of its liquid fermenting culture. The grifola frondosa facility planting new variety is preserved in China General Microbiological Culture Collection Center, with preservation number of CGMCC No.14354; the preservation date is July 19, 2017. The grifola frondosa new variety can resist low temperature and is high in planting cycle; the biological conversion rate is up to 40%; the space utilization rateof gridding facility planting is greatly improved, and the planting mode of time waste and work waste of a traditional earthing planting mode is avoided; the new variety can be planted semi-automatically and suitable for planting in northeast and north China areas; the preparation technology of the liquid fermenting culture of the grifola frondosa is low cost, short in cycle, rapid in mushroom generation, high in purity, few in pollution, and others.