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CN107815444A 一种用于ATP再生的底盘系统与应用 无效 - 驳回

一种用于ATP再生的底盘系统与应用
摘要：
本发明提供了一种用于ATP再生的底盘系统与应用，所述用于ATP再生的底盘系统包括以下5个酶：α-葡聚糖磷酸化酶、磷酸葡萄糖变位酶、磷酸葡萄糖异构酶、磷酸转酮酶以及乙酸激酶，此外还可以包括以下7个酶：转醛醇酶、转酮醇酶、核糖-5-磷酸异构酶、核酮糖磷酸3-差向异构酶、磷酸丙糖异构酶、果糖-二磷酸醛缩酶以及果糖-1,6-二磷酸酶；所述底盘系统可以与需要ATP的酶催化反应耦合，利用淀粉或麦芽糊精为能源，不添加辅酶，一锅式反应高效低成本地再生ATP，是经济、高效、稳定、可持续的ATP再生系统。
摘要（英）：

The invention provides a chassis system for ATP (Adenosine Triphosphate) regeneration and application. The chassis system for ATP regeneration comprises the following five enzymes: alpha-glucan phosphorylase, glucophosphomutase, phosphoglucose Isomerase, phosphoketolase and acetokinase. In addition, the chassis system can further comprise the following seven enzymes: transaldolase, transketolase,ribose-5-phosphate isomerase, ribulose-3 epimerase, triosephosphate isomerase, fructose-bisphosphate aldolase and fructose-1,6-bisphosphatase. The chassis system can be coupled to an enzymic catalyticreaction needing ATP, utilizes starch or maltodextrin as energy, is not added with any coenzyme, can efficiently regenerate the ATP at low cost through a one-pot reaction and is an economical, highlyefficient, stable and sustainable ATP regeneration system.

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