The invention discloses antioxidant pentapeptide from miichthys miiuy meat. The invention also discloses application of the pentapeptide to the preparation of antioxidant health care products. The antioxidant pentapeptide has the beneficial effects that the miichthys miiuy meat antioxidant pentapeptide, provided by the invention, has good clearing effects on 1,1-diphenyl-2-trinitrophenyl hydrazyl(DPPH) free radicals, hydroxyl radicals and superoxide anion free radicals; the pentapeptide can obviously improve the activity of intracellular superoxide dismutase (SOD) and glutathion peroxidase (GSH-Px); the contents of intracellular nitric oxide (NO) and malondialdehyde (MDA) can be obviously reduced; the HUVEC cell oxidization injury is induced by hydrogen peroxide to achieve the protectioneffect; the pentapeptide has the advantages of safety, nontoxicity, high anti-oxidization activity, easy digestive absorption and the like; the antioxidant pentapeptide can be used as a medicine, health care food and food additives.