The invention provides a membrane electrode assembly of a fuel cell. The membrane electrode assembly comprises a gas diffusion layer, a micro-porous layer, a catalysis layer and an electrolyte membrane which are successively overlapped; in a direction of an air flow path, the thickness of the micro-porous layer is gradually reduced, the thickness of the catalysis layer is gradually increased, andthe total thickness of the micro-porous layer is consistent with the total thickness of the catalysis layer. The invention also provides a production method of the membrane electrode assembly of the fuel cell. The membrane electrode assembly of the fuel cell has the functions of balancing the content of water in areas of a gas inlet and a gas outlet of the fuel cell, finally improving the stability of the fuel cell under various temperatures and humidities, improving durability, reducing the catalyst loading capacity, and the like.