The invention relates to a non-metallic bicycle frame structure and a manufacturing method thereof. The manufacturing method includes the step of preparing bicycle frame accessories which include a bicycle frame joint and a pipe fitting, wherein the bicycle frame joint is made of fiber reinforced engineering plastics and thermosetting or thermoplastic resin and includes a head pipe joint, a seat pipe joint, a five-way joint and a rear fork hook joint; the pipe fitting is made of one or more of fiber reinforced engineering plastics and thermosetting or thermoplastic resin and includes an inclined beam pipe, a seat pipe, a vertical fork pipe and a flat fork pipe; the step (2) of performing bicycle frame accessory surface treatment; the step (3) of sticking the bicycle frame joint to a pipe;and the step (4) of performing coating on a bicycle frame. A pipe joint type forming method is adopted for the whole bicycle frame, the material is light and high in strength, and the bicycle frame made by adopting the method is light and high in structure, has higher dimensional accuracy and is suitable for large-scale low-cost production.