The invention relates to a fermentation production method of oxidized coenzyme Q10 and high-content oxidized coenzyme Q10 prepared by the fermentation production method. The fermentation production method of the oxidized coenzyme Q10 is characterized in that in a fermentation process of a produced strain, redox potential ORP of fermentation liquid is controlled to be -50-300 mV, and the redox potential ORP of the fermentation liquid is preferably controlled to be 50-200 mV. According to the fermentation production method of the oxidized coenzyme Q10, ORP of the fermentation liquid is controlled so that the content of the oxidized coenzyme Q10 in the coenzyme Q10 produced from microorganisms can reach 96% or above, constitutes of the product are single, and aftertreatment is facilitated; and the oxidized coenzyme Q10 is more stable than reduced coenzyme Q10, and compared with coenzyme Q10 produced by fermentation in the prior art, the high-content oxidized coenzyme Q10 is less degradedin a living body. In addition, the fermentation method is high in valence.