The invention relates to a PSP (pancreatic stone protein) detection kit with insect cell expression PSP as a calibration product, belongs to the biotechnological field and discloses the process as follows: the purified insect cell expression PSP is used as the calibration product, an LFIA (lateral flow immunoassay) test strip is established, the fluorescence intensity of the test strip is determined with TRFIA (time-resolved fluoroimmunoassay), and the concentration of PSP in blood or liquids is detected. The method comprises the steps as follows: extracting RNA in gastric cancer cells (AGS),synthesizing cDNA, transferring recombinant Flag-PSP AcNPV plasmids into insect cells, purifying PSP as the calibration product, marking carboxyl modified particles, assembling the LFIA test strip andestablishing an automatic PSP concentration display system. The kit has the advantages of being high in sensitivity, high in specificity, convenient to operate and capable of rapidly diagnosing bacterial infection and sepsis.