The invention discloses a chicken meat polypeptide powder, and a preparation method and an application thereof. The preparation method includes steps of: raw material pretreatment, degreasing, controllable enzymolysis, high-temperature enzyme deactivation, separation, vacuum concentration and spray drying. According to the technical scheme, the raw materials are subjected to steps of cleaning andimpurity removal, and through processes of enzymolysis, separation, concentration, spray drying and the like, the chicken meat polypeptide powder is prepared. The product is rich in small-molecular polypeptides and amino acids, is nutritional and healthy, is safe and delicious and can satisfy demands of people on nutrients and taste. The preparation method reduces resource waste, can create highereconomic benefit to enterprises, is suitable for industrial production of chicken meat polypeptide powder and has excellent application prospect.