The invention discloses a benzopyrone dipolymer and an extracting method and an application of the benzopyrone dipolymer. The extracting method comprises the following steps: (1) culturing a strain ofmedium endophytic fungi aspergillus tubingensis DS37 of decaisnea insignis in a strain culture; (2) fermenting and culturing the strain obtained in the step (1) in a fermenting and culturing medium,extracting with petroleum ether, ethyl acetate and methyl alcohol for a plurality of times, mixing extracting solutions, filtering, recovering a mixed solvent, implementing vacuum concentration on a filtrate to obtain a coarse product of an extractum, and finally conducting column chromatography on silica gel to obtain the benzopyrone dipolymer. The prepared benzopyrone dipolymer has an excellenteffect on inhibiting staphylococcus aureus, and can be used in the technique of chemical control; the benzopyrone dipolymer is relatively strong in anti-A549 cell strain activity and provides a possibility for the development of the antitumor drug; and meanwhile, the benzopyrone dipolymer has a certain antioxidant activity.