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发明公开
CN108409850A 一种鱼源半乳糖凝集素CaGal重组蛋白的制备方法及其应用 无效 - 撤回

一种鱼源半乳糖凝集素CaGal重组蛋白的制备方法及其应用
摘要：
本发明公开了一种鱼源半乳糖凝集素CaGal重组蛋白的制备方法及其应用，属于水产动物分子生物学领域。本发明的技术方案要点为：以淇河鲫cDNA为模板，采用F/R为引物进行PCR扩增，PCR产物CaGal与载体pET-32a连接，构建pET-32a-CaGal重组表达载体；转化至大肠杆菌BL21(DE3)获得大肠杆菌表达菌株pET-32a-CaGal-BL21，即可表达半乳糖凝集素CaGal重组蛋白，经分离和纯化获得鱼源半乳糖凝集素CaGal重组蛋白。本发明制备的CaGal重组蛋白可保持其天然生物学活性，能够有效抑制嗜水气单胞菌等病原菌，抑菌效果明显，并且制备方法简单，可作为一种新型的鱼类免疫添加剂或抗菌药物。
摘要（英）：

The invention discloses a preparation method and application of a fish-derived galactose lectin CaGal recombinant protein, and belongs to the field of aquatic animal molecular biology. According to the technical scheme, the cDNA of Qihe crucian carp Carassius auratus is took as a template, F/R is used as the primer for carrying out PCR amplification, the PCR product CaGal is connected with a carrier pET-32a to construct pET-32a-CaGal recombinant expression vector; the vector is transformed into escherichia coli BL21 (DE3) to obtain the escherichia coli expression strain pET-32a-CaGal-BL21 which can express the galactose lectin CaGal recombinant protein, and the galactose lectin CaGal recombinant protein can be obtained through separation and purification. The galactose lectin CaGal recombinant protein can keep the natural biological activity, so that pathogenic bacteria such as aeromonas hydrophila can be effectively inhibited, the antibacterial effect is obvious, and the preparation method is simple and can be used as a novel fish immune additive or an antibacterial drug.

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