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发明公开
CN108410775A 一株高产维生素K2（MK-7）的纳豆芽孢杆菌及其应用 有权

一株高产维生素K2（MK-7）的纳豆芽孢杆菌及其应用
摘要：
本发明公开了一株高产维生素K2(MK-7)的纳豆芽孢杆菌及其应用，属于微生物领域。本发明的纳豆芽孢杆菌(Bacillus subtilis natto)ND-1-A27于2018年3月14日保藏于中国典型培养物保藏中心，保藏地址为中国武汉武汉大学，保藏编号为CCTCC NO:M 2018131。大幅度提高了维生素K2(MK-7)的产量，产量约达70mg/L，并且仅需要3天即可达到最大产量，大大缩短了发酵周期，节约时间成本，能够应用于工业化发酵生产且该菌株遗传稳定性良好，连续传代5代其产维生素K2(MK-7)产量基本稳定，维持在同一较高水平，能够作为进一步研究和开发的生产菌株。
摘要（英）：

The invention discloses bacillus subtilis natto for high yield rate of vitamin K2 (MK-7) and application thereof, and belongs to the field of microorganisms. The bacillus subtilis natto ND-1-A27 was collected into China Center for Type Culture Collection on March 14, 2018 in Wuhan University of China, and the collection number is CCTCC NO:M 2018131. The bacillus subtilis natto has the advantages that the yield rate of the vitamin K2 (MK-7) is greatly increased and is about 70mg/L, and the maximum yield rate can be reached by about three days; the fermenting cycle is greatly shortened, the timeis shortened, and the cost is reduced; the bacillus subtilis natto can be applied to industrialized fermenting production, and the genetic stability is good; after five generations are continuously transferred, the yield rate of the vitamin K2 (MK-7) is basically stable, and is maintained at the higher level; the bacillus subtilis natto can be used as a production bacterial strain for further study and development.

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