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发明公开
CN108504627A 人类黄韧带干细胞的提取方法 无效 - 撤回

人类黄韧带干细胞的提取方法
摘要：
本发明涉及一种人类黄韧带干细胞提取方法，该方法有以下步骤：1)取人类黄韧带组织，冲洗，剪碎；2)剪碎的组织用一型胶原酶将黄韧带消化；3)收集消化细胞，得到单细胞悬液；4)培养细胞，传代三次；5)用浓度为10μg/ml的纤连蛋白溶液筛选干细胞。该方法成本低，提取的黄韧带干细胞量大、存活率高、更易于在培养瓶内贴壁、细胞活性好。所得黄韧带干细胞纯度高，对人体无伤害，提取的黄韧带干细胞还可以为椎间盘组织工程提供一种种子细胞。
摘要（英）：

The invention relates to an extracting method for human ligamentum flavum stem cells. The method comprises the following steps: 1) taking human ligamentum flavum tissue to flush and cut into pieces; 2) digesting ligamentum flavum of the cut tissue with collagenase; 3) collecting digestive cells to obtain single-cell suspension; 4) culturing the cells to passage for three times; and 5) screening the stem cells by a fibronectin solution with concentration being 10 mug/ml. The method is low in cost, and the extracted human ligamentum flavum stem cells are great in number, are high in survival rate, are liable to adhere to a culture bottle, and are good in cell activity. The obtained ligamentum flavum stem cells are high in purity, are free of harm on a human body, and the extracted ligamentumflavum stem cells further can provide a seed cell for intervertebral disc tissue engineering.

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