The invention belongs to the technical field of microbial fermentation and particularly relates to a fermentation method capable of increasing bacillus produced protease. In a 10-30 L fermentation system, the enzyme activity of the bacillus produced protease is improved by 50%-60% through large-dosage inoculation of 30%-40% (v/v), fermentation control and material supplement, so that the fermentation method has good application prospects.