The invention discloses an Ni-63 radioactive flake source electrodeposition method. The method adopts electrodeposition liquid centering on nickel sulfate to prepare a 63Ni radioactive source. The method mainly includes the following steps: (1) preparing the electrodeposition liquid of nickel sulfate; (2) performing pre-processing and pre-electrodeposition on stainless steel substrate; (3) adjusting each parameter of an electrodeposition instrument, and performing the electrodeposition to prepare the 63Ni radioactive source. According to the Ni-63 radioactive flake source electrodeposition method, the 63Ni radioactive source nickel layer obtained by this scheme is compact and uniform, has metallic luster, and is combined closely with the substrate and not easy to fall off.