The invention provides a preparation method of a palladium-carbon catalyst for imipenem. The preparation method comprises the following steps of refluxing activated carbon by a low-concentration glycine solution, and impregnating by an aqueous solution of water-soluble acetate; adding sodium nitrite into a precursor solution of palladium chloride, dripping into the treated activated carbon slurry,impregnating, adjusting the pH (potential of hydrogen) value to 8 to 9 by an alkaline solution adjusting system after impregnating, and filtering; pulping the obtained filter cake by purified water,and adding sodium borohydride to reduce, so as to obtain the palladium-carbon catalyst. The preparation method has the advantages that the activated carbon is treated by the low-concentration glycinesolution, and is impregnated by the aqueous solution of water-soluble acetate, so that the surface chemical structure is improved, and the pollution caused by treatment of the activated carbon by thetraditional nitric acid treatment method is avoided; by adjusting the modified active component solution to prepare the chelating-state palladium ions, the purpose of controlling the activity and selectivity of the catalyst is realized; the technology is simple, and the preparation method is suitable for industrialized amplification production.