The invention relates to a release nut connection unlocking device, which is used for connecting and unlocking two separating bodies, comprising a separating nut, a screw rod, a collecting cylinder, aloading nut, an elastic member and a locking sleeve. The separating nut connection unlocking device comprises a separating nut, a screw rod, a collecting cylinder, a loading nut, an elastic member and a locking sleeve. The screw passes through the elastic member and is axially limited by a locking sleeve; When the two separators are connected, the screw runs through the two separators, the lowerend of the screw is connected with the separation nut, and the upper end is located in the collecting cylinder; The screw is locked by a loading nut; When the two separating bodies are unlocked, the screw is separated from the separating nut by the separating action of the separating nut, and the axial interaction force with the collecting cylinder is maintained under the action of the elastic member. The release nut is separated from the separating nut by the separating action of the separating nut. As that load nut does not bear the force exerted by the elastic member through the locking sleeve, the pre-tightening force exerted on the screw by the loading nut is more accurate, and the reliability of the connection unlock device of the separation nut is improved.