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发明公开
CN109182303A 一种巴伦葛兹类芽孢杆菌β-N-乙酰氨基葡萄糖苷酶及其编码基因与应用 有权

一种巴伦葛兹类芽孢杆菌β-N-乙酰氨基葡萄糖苷酶及其编码基因与应用
摘要：
本发明公开了一种巴伦葛兹类芽孢杆菌β-N-乙酰氨基葡萄糖苷酶及其编码基因与应用。本发明提供的β-N-乙酰氨基葡萄糖苷酶PbNag39的酶学性质优异，对几丁二糖的比酶活为28.3U/mg，水解几丁寡糖的终产物均为N-乙酰氨基葡萄糖。将该β-N-乙酰氨基葡萄糖苷酶与几丁质酶协同水解几丁质粉末，可高效制备得到N-乙酰氨基葡萄糖。本发明的β-N-乙酰氨基葡萄糖苷酶具有比酶活高、热稳定性好和水解特性优异的特点，能在较广泛的pH范围内稳定并发挥催化作用，在几丁质转化中具有重要的应用价值。
摘要（英）：

The invention discloses a Beta-N-acetylglucosaminidase of Paenibacillus barungensis, an encode gene thereof and an application of beta-N-acetylglucosaminidase. The provided Beta-N-acetylglucosaminidase PbNag39 is excellent in the enzymatic property, and has a specific activity against chitosan is 28.3 U/mg, and the final products of hydrolysis of chitosan oligosaccharides are N-Acetyl glucosamine.The Beta-N-Acetylglucosaminidase and chitinase can hydrolyze chitin powder synergistically and obtain N-Acetyl glucosamine. The Beta-N-acetylglucosaminidase PbNag39 has the characteristics of high specific activity, good thermal stability and excellent hydrolysis characteristics. It can stabilize and play a catalytic role in a wide range of pH, and has important application value in chitin conversion.

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