The invention discloses a method for producing maize sweet and glutinous kernelidioplasm. The method comprises the following steps:site-directed mutation is carried out onan Sh2 gene and a Wx gene ofa maize receptor, and double homozygous mutated maize with mutated Sh2 gene and Wx gene is selected; site-directed mutation is carried out on the Wx gene in the maize receptor, single mutation maize with amutatedWx gene is selected. The double homozygous mutanted maize is hybridized with the single mutation maize with the mutated Wx geneto obtain a hybrid generation. Self-crossing is carried out on the next generation, maize kernel with higher sweetness and higher glutinosity is obtained. The hybrid generation is the sweet and glutinous maizeisioplasm, which has a wide market prospect.