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发明公开
CN109486797A 一种促进蛋白酶高效分泌的方法 有权 转让

一种促进蛋白酶高效分泌的方法
摘要：
本发明公开了一种促进蛋白酶高效分泌的方法，属于基因工程技术领域以及酶工程技术领域。此方法通过将氨基酸序列如SEQ ID NO.1所示的碱性蛋白酶的第69位丙氨酸突变为精氨酸或在将氨基酸序列如SEQ ID NO.1所示的碱性蛋白酶的第69位丙氨酸突变为精氨酸的基础上，进一步将第376位苏氨酸突变为异亮氨酸，大大提高了碱性蛋白酶的活性，对于进一步推动碱性蛋白酶的大规模工业化应用、降低产业成本具有重大的意义。
摘要（英）：

The invention discloses a method for promoting the efficient secretion of protease, belonging to the technical fields of genetic engineering and enzyme engineering. According to the method, 69-site alanine of alkaline protease with an amino acid sequence represented by SEQ ID NO.1 is mutated into arginine, or 376-site threonine is further mutated into isoleucine on the basis that 69-site alanine of alkaline protease with an amino acid sequence represented by SEQ ID NO.1 is mutated into arginine, so that the activity of alkaline protease is greatly improved, and the method has great significances to the further promotion of the large-scale industrial application of alkaline protease and the lowering of the industrial cost.

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