The invention belongs to the technical field of amino acid production, and discloses an amino acid fermentation culture medium. The culture medium comprises a fermentation culture medium A and a fermentation culture medium B. The fermentation culture medium A and the fermentation culture medium B are used separately, the amino acid is glutamic acid, and the fermentation culture medium B is obtained by adding inositol and glycerinum on the basis of the fermentation culture medium A. By optimizing the culture medium, the glutamic acid fermentation process is more stable, the cell contraption andthe glutamic acid yield are increased, the glutamic acid extracting cost is lowered, and the good application prospect is achieved.