The invention relates to a mesoporous carbon-supported noble metal catalyst as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing a carbon carrier precursor, a soft templating agent, a noble metal precursor and a solvent, then evaporating the solvent, and performing heat treatment and carbonization treatment to obtain the catalyst. According to the preparation method, through a synergistic effect of the carbon carrier precursor, the soft templating agent and the noble metal precursor and effective combination of the steps of solvent evaporation and heat treatment, distribution of noble metal particles on the prepared catalyst and uniformity of the sizes are improved; the carbon-supported noble metal catalyst prepared by the preparation method provided by the invention has excellent electrochemical performance; when Pt is selected as a noble metal, the electrochemical active area of a cyclic voltammetry curve thereof is 73.12-103.53m<2>/g, while the electrochemical active area of the cyclic voltammetry curve of the current commercial JM40%Pt/C is 65.27m<2>/g.