The invention discloses a propagation release card, a folding type propagation release card and a use method thereof. The upper part of a propagation release card body is provided with an opening area, the lower part of the propagation release card body is provided with an egg sticking area for sticking parasitic eggs of anastatus japonicus, the propagation release card body is also provided witha quick tearing line leading to a hanging hole, and the quick tearing line penetrates through the gravity center of the propagation release card body; a folding type propagation release card body comprises a first card body and a second card body, which are the propagation release cards, the quick tearing lines of the two card bodies are connected, and the two card bodies are folded in half alonga folding line; the use method of the propagation release card comprises the preparation of the propagation release card and the field application of the propagation release card. According to the invention, a prior propagation release card is improved, the suspension stability of the card body on branches of a fruit tree can be improved, and the release process of the anastatus japonicus is effectively simplified.