The present invention provides a bicycle vehicle and single item fatigue test analysis platform and an application method thereof. The platform comprises a platform base frame, a vehicle wheel flexible fixing mechanism, a vehicle wheel assistant regulation mechanism, a pedal drive mechanism, a vehicle wheel rigid fixing mechanism, a vehicle handlebar vertical loading mechanism and a saddle multi-directional loading mechanism. The method comprises the steps of: putting a bicycle on the vehicle wheel assistant regulation mechanism and regulating the bicycle position to allow a pedal to enter therange of the pedal drive mechanism to fix the pedal with a clamping plate in the pedal drive mechanism; connecting the vehicle handlebar vertical loading mechanism with a vehicle handlebar, and connecting the saddle multi-directional loading mechanism with a saddle; starting the motor of the pedal drive mechanism, outputting down force by a hydraulic cylinder to simulate the true working state ofthe bicycle; shutting down the motor and unloading the hydraulic cylinder after the operation is finished, and checking the vehicle damaging condition; and removing the vehicle wheel flexible fixingmechanism, replacing the vehicle wheel rigid fixing mechanism, applying load to the hydraulic cylinder again, unloading the hydraulic cylinder after the loading is finished, and checking the damagingconditions of key parts of the bicycle.