The invention discloses an F3 polypeptide targeted nano organic metal frame material (nMOFs) and a preparation method thereof. A transition element Zr and an organic ligand BDC-N3 are synthesized intoa nano-carrier UIO-66-N3, and then two PEGs of which the tail ends are modified by alkynyl are loaded on the surface of the UIO-66-N3 through a click chemical reaction, thereby avoiding the aggregation between nanoparticles, and increasing the long circulation effect of the nanoparticles; then an F3 polypeptide is conjugated on the surface of the UIO-66-N3 to give the UIO-66-N3 targeting. In thewhole chemical transformation process, the structure of a framework of the UIO-66-N3 is retained and loaded with DOX to prepare a fluorescent imaging agent for treatment, and the nanoparticles of thesynthesized DOX/UIO-66-PEG-F3 have the effects of treatment and targeting. The F3 polypeptide targeted nano organic metal frame material (nMOFs) has potential clinical application value, and providesmore powerful technical support for the treatment of malignant tumors.