The invention relates to the field of organic chemistry and pharmaceutical chemistry, in particular to aryl isoquinooxazole quaternary ammonium salt compounds as shown in a formula I, a preparation method and an application thereof in preparing a drug for treating tumors or cancers. The aryl isoquinooxazole quaternary ammonium salt compounds play a relatively good role of inhibiting growth of multiple myeloma and lymphoma cells in vitro. In addition, the compounds also have certain inhibiting activity on lung cancer, colon cancer, breast cancer, liver cancer and prostate cancer cells and the like. The compounds have wide anti-tumor effect.