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发明公开
CN110129254A 一种以Percoll为介质分离方斑东风螺颗粒细胞和透明细胞的方法 审中-实审

一种以Percoll为介质分离方斑东风螺颗粒细胞和透明细胞的方法
摘要：
本发明公开了一种以Percoll为介质分离方斑东风螺颗粒细胞和透明细胞的方法，属于方斑东风螺血细胞分选技术领域。本发明的技术方案要点为：取15mL离心管，加入3mL体积分数为38%的Percoll溶液和2mL方斑东风螺血细胞悬液，在1300g 4℃离心45min，溶液分为上下两层和上下两层的交界层，再取一支新的15mL离心管，加入3mL体积分数为35%的Percoll溶液和1mL上下两层的交界面溶液，再次1300g 4℃离心45min，分别收集上层溶液和下层溶液，最终实现方斑东风螺颗粒细胞和透明细胞的分离。本发明分选过程简单、方便、易操作、特异性强且成本低廉。
摘要（英）：

The invention belongs to the technical field of babylonia areolata hemocyte separation and discloses a method for separating babylonia areolata granular cells and clear cells by Percoll serving as medium. The technical scheme is characterized in that the method includes steps: taking a 15mL centrifuge tube, adding 3mL Percoll solution in volume fraction of 38% and 2mL of babylonia areolata hemocyte suspension, and centrifuging for 45min under conditions of 1300g and 4 DEG C; after solution is layered into upper and lower layers and an interface layer between the upper and lower layers, takinganother new 15mL centrifuge tube, adding 3mL Percoll solution in volume fraction of 35% and 1mL of interface solution of the upper and lower layers, centrifuging for 45min under conditions of 1300g and 4 DEG C, and collecting upper-layer solution and lower-layer solution respectively to finally realize separation of babylonia areolata granular cells and clear cells. A separation process is simple,convenient, easy in operation, high in specificity and low in cost.

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