The invention relates to a prefabricated high-pressure airbag isolation pile structure with a grouting function and a construction method. The structure comprises a prefabricated high-pressure airbagpile body, an automatic adjusting system and a grouting system. The structure has the following beneficial effects: the prefabricated high-pressure airbag isolation pile is adopted to replace a traditional isolation pile; a high-pressure airbag is distributed in soil near the bottom of a foundation pit by a certain depth range, high-pressure gas is injected in the high-pressure airbag to form expansion pressure to change a peripheral stress field to cut off a stress transfer path, so that the horizontal traction effect of the rigid isolation pile is eliminated, and the displacement deformationgenerated by self deformation of the traditional isolation pile is made up; and the inflation expansion of the prefabricated high-pressure airbag is used for assisting the prefabricated pile to resist the deformation of the soil to realize real-time adjustment of the displacement of deep soil, so that the effect is better compared with the traditional isolation pile, the displacement deformationof the traditional isolation pile due to insufficient rigidity is made up, and the displacement of the deep soil on the outer side of an active area is reduced.