The invention discloses a membrane emulsification assembly for producing micron-sized micro-spheres and application of the membrane emulsification assembly for producing the micron-sized micro-spheres. The membrane emulsification assembly comprises a membrane pipe jacket with two ends closed, a membrane pipe with two ends closed and an embedded pipe with two ends closed, wherein membrane holes areformed in the membrane pipe, the embedded pipe is fixed to the interior of the membrane pipe, a gap between the embedded pipe and the membrane pipe is circular, the membrane pipe is sleeved with themembrane pipe jacket through fixing parts, a continuous phase inlet is formed in one end of a gap between the membrane pipe and the embedded pipe, an emulsion outlet is formed in the other end of thegap between the membrane pipe and the embedded pipe, a dispersion phase inlet is formed between the two ends of the membrane pipe jacket, and the membrane emulsification assembly can be used for producing the micron-sized micro-spheres carried with medicines or the micron-sized micro-spheres carried with no medicines. By means of the membrane emulsification assembly for producing the micron-sizedmicro-spheres and application of the membrane emulsification assembly for producing the micron-sized micro-spheres, the technical problem of wide particle size distribution of micro-spheres with largeparticle size is solved, the produced micro-spheres have good repeatability, moreover, the particle size is uniform, particle size distribution can stabilize at 1.0 or below, micro-spheres with uniform particle size of 60-200 [mu]m can be produced, the technology is simple and convenient, and the cost is low.