The invention discloses a fruit and vegetable preservative containing cedar essential oil, a preparation method and an application thereof. The fruit and vegetable preservative containing the cedar essential oil is composed of the following raw materials: by weight, 1L of water contains 60 to 80 g of the cedar essential oil, 30 to 60 g of a salvia sclarea extract, 150 to 280 g of an emulsifier, and 15 to 30 g of Arabic gum. The cedar essential oil and the salvia sclarea extract contained in the preservative have strong inhibitory effect on microorganisms, and are important sources of natural preservatives.